SOLVED: Why is the 16S rRNA gene sequenced and not another gene? What are housekeeping genes? What is multilocus sequence analysis (MLSA)? What is a phylogenetic tree? What is dDDH and ANI? Why is it necessary to determine these values?
SOLVED: Why is the 16S rRNA gene sequenced and not another gene…
Sep 8, 2021
numerade.com
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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16S rRNA gene is widely used in phylogenetic and microbiome research, however, a study published in Microbiome suggests it has a weak level of concordance with core genome phylogenies. Read the paper: https://bit.ly/3cn2o2x | BMC
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Video: Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
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