Engineering the L-tryptophan metabolism for efficient de novo …
Oct 16, 2024 · Here we characterized and engineered the less-studied L-tryptophan pathway and IET biosynthesis in the baker’s yeast Saccharomyces cerevisiae, with the goal of investigating microbial …
Biosynthetic Pathways of Tryptophan Metabolites in Saccharomyces …
Despite a slight inhibition in yeast growth, the overall production of tryptophan metabolites notably increased. In place of peptone, tryptophan was expected to serve as a nitrogen source.
Saccharomyces cerevisiae tryptophan degradation
S. cerevisiae degrade the aromatic amino acids (tryptophan, phenylalanine, and tyrosine) and the branched-chain amino acids (valine, leucine, and iso-leucine) via the Ehrlich pathway …
Metabolic Engineering and Fermentation Process Strategies for L-Tryptophan …
Apr 12, 2019 · Based on the literature reported during the last decade and the recent advances, this review systematically describes the genetic engineering modification and fermentation optimization …
Predictive engineering and optimization of tryptophan metabolism in yeast …
Nov 29, 2019 · Based on a single high-throughput data-generation iteration, this study highlights the power of combining mechanistic and machine learning models to enhance their predictive power and …
High levels of Tryptophan reduce cell wall or membrane stress …
Aug 13, 2021 · Tryptophan (Trp) is a proteinogenic aromatic amino acid; however, high levels of Trp are toxic in animals and yeast with unknown mechanisms. Previously, we suggested that aromatic …
Enzymatic Basis for Overproduction of Tryptophan and Its …
The DAHP synthetase activity of Hansenula polymorpha was subject to additive feedback inhibition by phenylalanine and tyrosine but not by tryptophan. The synthesis of DAHP synthetase in this yeast …
Substrate-induced differential degradation and partitioning of the two tryptophan …
Apr 1, 2022 · Tryptophan is a relatively rare amino acid whose influx is strictly controlled to meet cellular demands. The yeast Saccharomyces cerevisiae has two tryptophan permeases, namely Tat1 (low …
Engineering Saccharomyces cerevisiae for the de novo Production of …
Mar 16, 2023 · In this study, conceptual de novo production of a diverse range of halogenated tryptophan and tryptamine derivatives was achieved in the baker's yeast S. cerevisiae by the …
Optimizing Fermentation Strategies for Enhanced Tryptophan …
Nov 2, 2024 · Tryptophan is an essential aromatic amino acid widely used in the pharmaceutical, agricultural, and feed industries. Microbial fermentation, mainly using Escherichia coli, has become …